Abstract: BRCA1 coordinates cellular responses to DNA damage. It functions as a co-repressor of GADD45atranscription through interactions with a DNA-binding protein termed ZBRK1. Our goal is to develop a biosensor system to visualize transcription control by ZBRK1 and BRCA1 in single living and/or fixed cells. The rationale is to use integrated DNA binding sites to obtain real time, multiplex-based data. The reportable outcomes for the period are: 1) UAS and ZREarray-bearing plasmids have been constructed; 2) Transient and stable reporter expression have been demonstrated; 3) Stable cell lines with G20, G40 and Z32 are on line for studies;4) Construction of fluorescent GAL4-DBD and ZBRK1 fusion protein has been achieved, and BRCA1 derivatives are in progress. When operational, this system will document real time nuclear dynamics of ZBRK1/BRCA1-dependent chromatin modification systems, as cells mount transcriptional responses to genotoxins.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual rept. 15 Jul 2004-14 Jul 2005 |
| Pages: |
8 |
| Report Date: |
AUG 2005 |
| Contract Number: |
W81XWH0410700 |
| Report Number: |
A334264 |
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