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Abstract:
The measurement of free iron in hemoglobin solutions as well as the measurement of total hemoglobin-bound iron are important validation assays for large-scale production of hemoglobin-based oxygen carriers. In addition, the detection of iron released from hemoglobin by oxidation or reduction reactions provides information about the stability of iron in heme. These stability measurements may be important in the characterization of structurally modified hemoglobins and can be conducted using spectrophotometric assays based on ferrozine, a chelator of ferrous iron. Techniques for the measurement of free iron in hemoglobin solutions, total hemoglobin-bound iron, and iron released from hemoglobin are described in detail.... Hemoglobin, Hemoglobin solutions, Hemoglobin-based oxygen carriers, Blood substitutes, Iron, Oxidation, Reduction, Ferrozine, Chromophores.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Pages: |
26 |
| Report Date: |
17 FEB 93 |
| Report Number: |
A305662 |
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