Military personnel are vulnerable to many biological agents, such as bacteria, toxins and viruses, in the field. These agents must be detected rapidly to allow the prompt treatment of infected personnel. The proposed technique employs a piezoelectric detector with built- in control and sensor zones which was seen to offer better than 1000 fold rejection of such matrix proteins as albumin and immunoglobulin G while detecting 10 nanograms of cholera toxin B-subunit per milliliter of solution. The response time was less than 5 minutes. The report details the study of several alternative chemistries for the covalent attachment of antibody to the sensor - all of which offer binding densities between 200 and 600 picogram per millimeter binding densities. Several candidate substrate materials were investigated and a temperature stable orientation for SHAPMs was located. Sensor regeneration was demonstrated using glycine-buffered saline.
Final rept. 15 Mar-14 Sep 95
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