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Abstract:
Quantification of HIV-1 RNA in human plasma and molecular biological analysis of viral replication and turnover promise to provide unique insights into AIDS pathogenesis and hasten antiretroviral therapy and vaccine research efforts. We evaluated quantitative competitive polymerase chain reaction (QC- PCR) and branched DNA (bDNA) signal amplification assays for plasma viral RNA in 152 HIV-1 positive individuals at all stages of illness and in 12 patients before and after initiating zidovudine therapy. Eighty-six percent of patients had bDNA values above the 10(exp 4) RNA Eq/ml assay sensitivity cutoff. bDNA values were significantly correlated with plasma viral RNA levels determined by QC-PCR (Spearman rank correlation, r = 0.89), infectious plasma virus titers (r = 0.72), p24 antigen (r = 0.51), immune complex dissociated p24 antigen (r = 0. 56), and CD4+ lymphocyte counts (r = 0.72; p<0.0001 for all comparisons). Plasma viral RNA determinations by bDNA and QC-PCR assays were quantitatively similar in the range of 10(exp 4) to 10(exp 7) RNA molecules/ml (log bDNA = 0.93 + 0. 80(*) log QC-PCR; R2 = 0.81, p<0.0001) and declined identically following the institution of zidovudine therapy (68-73% decrease from baseline).
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual rept. 22 Jun 93-21 Jun 94 |
| Pages: |
46 |
| Report Date: |
20 JUL 94 |
| Contract Number: |
DAMD17-93-C-3146 |
| Report Number: |
A235382 |
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