|
Abstract:
This report describes the utilization of the isolated perfused porcine skip flap (IPPSF) to characterize the cutaneous toxicity of sulfur mustard (XHD) and lewisite (XL). The IPPSF is an humane, alternative, in vitro, animal model consisting of an isolated perfused skin preparation with intact and viable epidermis and dermis in association with a functional microcirculation. Task 1 of the present contract was to characterize the dose-response profile of both vesicants in 53 IPPSFs using biochemical, physiological, and morphological (light and transmission electron microscopy) endpoints. Twenty-four IPPSFs were treated with 200 ul of either 5.0, 2.5, 1.25, 0.2, 0.14, or 0.07 mg/ml XL, and 29 IPPSFs were treated with 200 micron l of either 10.0, 5.0, 2.5, 1.25, 0.5 or 0.2 mg/ml XHD. At least four replicates were performed per compound, and four untreated IPPSFs served as controls. Experiments lasted 8 hours. Both XL and XHD resulted in a reduction in the cumulative glucose utilization and an increased vascular resistance as compared to controls. The high-dose groups for both agents consistently showed a marked alteration in both parameters.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Rept. for 30 Sep 87-1 Apr 90 |
| Pages: |
144 |
| Report Date: |
01 APR 90 |
| Contract Number: |
DAMD17-87-C-7139 |
| Report Number: |
A229922 |
|
|
|
|
|
Keywords relating to this report:
Email This Abstract
