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Abstract:
Further investigations of the endogenous metabolism and survival of non-spore-forming anaerobic bacteria Zymomonas anaerobia and Peptococcus prevotii under conditions of starvation are reported. To permit studies with cells whose growth has been halted by exhaustion of a specific metabolite, the nutritional requirements of both organisms have been examined. The results obtained are described in the body of the report. In regard to growth and subsequent starvation it was found that there was no change in the gel electrophoresis pattern of P. prevotii thus indicating that the constancy of its total protein content under these conditions does not mask a preferential degradation and synthesis of individual proteins. As starvation progresses the ability of cells to produce ATP in response to a pulse of an energy-yielding substrate declines. Glucose, and to a much lesser extent ethanol, gives rise to ATP in starved cells of Z. anaerobia; pyruvate (which is decarboxylated by a non-energy-yielding reaction) does not. Starvation does not appear to impair this ability until the cells have been held without carbon and nitrogen for periods greater than 7 days. The slide culture technique as a measure of viability in this organism has been re-examined. A correlation does exist between ability to form micro-colonies on slide culture and intracellular ATP levels. On prolonged starvation, however, cells having the ability to form only very few colonies in slide culture, still possess unimpaired ability to produce ATP from glucose. (Author)
| Description: |
Final technical rept. May 67-Apr 68 |
| Pages: |
37 |
| Report Date: |
APR 1968 |
| Contract Number: |
DAJA3767C0567 |
| Report Number: |
0181676 |
Report Unavailable |
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