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Abstract:
The focus of the research project supported by this grant is to study the microbial ecology of bacterial populations involved in the degradation of environmental pollutants. Specifically, the purpose of this research is to further the knowledge base concerning the dynamics of gene expression within soil environments through the use of modern differential display techniques to study microbial gene expression and dioxin degrading activity associated with Agent Orange contaminated soils. Biodegradation of the herbicide Agent Orange probably involves a multitude of different microorganisms having diverse potentials for metabolic transformations of 2,4-dichlorophenoxyacetic acid (2,4, 5-trichlorophenoxyacetic acid (2,4,5-trichlorophenoxyacetic acid (2,4,5-T), and associated polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD)/DBF). Because of the complexity of microbially mediated degradation of Agent Orange constituents, a scheme was devised consisting of two-primary objectives: (1) to study the capacity of environmental bacteria to facilitate the biodegradation of 2,4-D, 2,4,5-T, and DD/DBF. (2) to identify and examine genes necessary for the degradation of these pollutants using both differential display reverse transcriptase PCR (DDTR-PCR) and other modern PCR derived techniques.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Final rept. 1 Aug 95-31 Jul 98 |
| Pages: |
5 |
| Report Date: |
JUL 1998 |
| Contract Number: |
F49620-95-1-0400 |
| Report Number: |
A120753 |
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Keywords relating to this report:
