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Refractive Index Determination of Biological Particles

Authors: Sean J. Hart; Tomasz A. Leski; NAVAL RESEARCH LAB WASHINGTON DC
Abstract:
Refractive indices of biological particles have been determined using a new quantitative method based upon phase contrast immersion spectrometry. The new technique is sensitive, accurate, and requires only an optical transmission spectrophotometer and index matching fluids. The method was developed and tested using colloidal silica standard samples. Refractive indices for bacterial spores have been determined, and the results compared favorably with available data in the literature.

Limitations: APPROVED FOR PUBLIC RELEASE
Description: Memorandum rept.
Pages: 12
Report Date: 31 JUL 2006
Report Number: A081454
Keywords relating to this report:
*PARTICLES
*REFRACTIVE INDEX
BACTERIA
COLLOIDS
LIGHT SCATTERING
LIQUIDS
OPTICAL PROPERTIES
SPORES
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