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Abstract:
Unlike other major common cancers, no major tumor genes have been reported in prostate cancer, although this disease is the most frequently diagnosed cancer and the second leading cause of cancer death in American men. Identifying tumor related genes is still one of the most significant areas in prostate cancer research. We have proposed to identify the genes that are involved in prostate cancer. One type of such gene are tumor suppressor genes. In the second year of this project, we continued our effort in characterizing tumor suppressor genes in prostate cancer. Using the methods of tissue microdissection and deletion mapping, we further defined a region of deletion, that indicates the existence of novel tumor suppressor gene, at the q21 band of chromosome 13. We found that some human tumors have homozygous deletion at 13q21. In addition, the LNCaP cell line and the PC-82 xenograft of prostate cancer have hemizygous deletion at 13q21, and the region of deletion is defined to 2.8 megabase. We are currently identifying the most likely candidate tumor suppressor gene from 1 3q21. We also used polymerase chain reaction (PCR)-single strand conformational polymorphism (SSCP) and direct sequencing methods to examine mutations of PTEN in high grade primary tumors and metastases of prostate cancer. We found that PTEN mutation is significantly more frequent in high grade prostate cancers, indicating that PTEN is more likely involved in the progression of prostate cancer.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual rept. 1 Aug 1999-31 Jan 2001 |
| Pages: |
15 |
| Report Date: |
FEB 2001 |
| Contract Number: |
DAMD17-98-1-8636 |
| Report Number: |
A051893 |
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